A new apparatus for preparative gel electrophoresis.

paratus is the dimension of the elution cham ber; if a large chamber is employed, dilution of solute occurs thus resulting in broadening of the elution patterns, while with a narrow chamber elution is frequently interrupted by the swelling of gel during operation. In many cases, either upward or downward elution has been employed and this leads to com plication of the apparatus. This communi cation describes a simple apparatus designed to circumvent these problems. The efficiency of the present apparatus was examined using monomer-polymer mixture of bovine serum albumin (Sigma Chemical Co., Ltd.) and crude ovalbumin (Nakarai Chemicals, Ltd.). The apparatus is composed of upper buffer reservoir (A), column (B), lower buffer re servoir (C), head pressure equilibration column (D) and elution buffer reservoir (E) (Fig.1). The column is connected to the upper and lower buffer reservoirs by means of joints. In order to avoid complexity of the apparatus, a horizontal elution system was adopted. The elution chamber (F), separating resolving